Trabajo presentado en el International Congress on Invertebrate Pathology and Microbial Control - 53rd Annual Meeting of the Society for Invertebrate Pathology, celebrado de forma virtual del 28 de junio al 2 de julio de 2021
Expanding the available biological control agents to fight against aerial grapevine pests can provide new strategies in both IPM and organic viticulture. Current application technology supports the implementation of entomopathogenic nematodes (EPNs) against aerial pests. We hypostatized that by selecting the best combination of EPN-adjuvant, we can enhance EPN viability and persistence on grapevine leaves, and herewith apply EPN against aerial grapevine pests. The aim of this study was to screen for the best adjuvant-EPN mix to ensure high survival, viability, and adherence on leaves. The compatibility of five commercial adjuvants approved for grapevine application (Multi-US, MaxiMix, Dash HC, Nu-film-17 and Adrex) and four EPN populations (Heterorhabditis bacteriophora VM-21, Steinernema carpocapsae ALL, S. feltiae RM-107 and S. feltiae Koppert) were tested by exploring the maximum adjuvant recommended field dosage concentration. In laboratory experiments, we investigated the survival of infective juveniles (IJs) after 4 and 24 h exposure to each adjuvant. Thereafter, we tested their infectivity against the model insect Galleria mellonella (Lepidoptera: Pyralidae). Adherence to leaves and survival was determined by spraying the IJs-adjuvant combination on grapevine leaves and establishing the leave coverage and their survival after 1 h. Overall, all the EPN populations were compatible with all the adjuvants, except for H. bacteriophora-Adrex combination. Also, most of the adjuvants provided similar to control leave coverages, maintaining IJs survival ~80% after 1 h. Additional studies are needed to increase the grapevine leave coverage by EPN. These promising results bring new opportunities to apply EPN against aerial grapevine pests.