Expression of the Erythrocyte Antigen Henshaw (He; MNS6): Serological and Immunochemical Studies
- Resource Type
- Authors
- Christine Lomas-Francis; C.A. Green; Geoff Daniels; Michelle Yacob; V. Chen; V. Hare; Y.C. Ho; J. Shen; E. Smart; M. E. Reid; R. Batts
- Source
- Vox Sanguinis. 68:183-186
- Subject
- Isoantigens
Erythrocytes
Hemagglutination
medicine.drug_class
Immunoblotting
Population
Monoclonal antibody
Serology
Antigen
medicine
Humans
Glycophorin
Serologic Tests
education
education.field_of_study
biology
Antibodies, Monoclonal
Hematology
General Medicine
Virology
Molecular biology
Red blood cell
medicine.anatomical_structure
biology.protein
MNSs Blood-Group System
Antibody
- Language
- ISSN
- 1423-0410
0042-9007
Production of murine monoclonal antibodies to the low prevalence MNS antigen Henshaw (He; MNS6) has enabled more detailed study of this antigen. Using these directly hemagglutinating anti-He, red blood cells (RBCs) from 1695 people of African origin were screened in the USA and England. The prevalence of He+ samples among these donors was 2.1%. In Natal, blood samples from 1218 black donors were screened with rabbit anti-He. The prevalence of He+ donors in this population was 7.0%. Immunoblotting confirmed that the He antigen is carried on an erythrocyte membrane component with a molecular mass that is indistinguishable from glycophorin B. Hemagglutination and immunoblotting demonstrated that ten of 56 He+ samples tested more extensively had a reduced expression of the He antigen. The majority of He+ RBCs were S+; those He+ RBC samples that were S-s+ more frequently had a weakened expression of He.