Additional file 1 of Identification of a novel gene signature predicting response to first-line chemotherapy in BRCA wild-type high-grade serous ovarian cancer patients
- Resource Type
- Authors
- Buttarelli, Marianna; Ciucci, Alessandra; Palluzzi, Fernando; Raspaglio, Giuseppina; Marchetti, Claudia; Perrone, Emanuele; Minucci, Angelo; Giac��, Luciano; Fagotti, Anna; Scambia, Giovanni; Gallo, Daniela
- Source
- Subject
- Language
Additional file 1: Figure S1. Pathology-based estimates of the percentage of tumor cells were evaluated from hematoxylin-eosin-stained slides. Only samples with higher than 70% of tumor purity were analyzed. Figure S2. a) Representative pictures of primary HGSOC cells stained using cytokeratin 7 (CK7) and TE-7 antibodies. The negligible staining for TE-7 (arrowhead) confirmed the absence of stromal fibroblast overgrowth in the primary cell lines generated. b) Morphology of established HGSOC cell lines and representative images of CK7 immunohistochemical staining. Table S1. Clinicopathological features of HGSOCs used for primary cell line isolation. Table S2. List of primers DeltaGene (Fluidigm) used in RT-qPCR. Table S4. Top DEGs in resistant vs sensitive HGSOC patients (downregulated genes). Top DEGs in resistant vs sensitive HGSOC patients (upregulated genes). Table S5. IC50 values of anticancer drugs against primary and commercially available HGSOC cell lines.