Nucleosomes, the basic structural units of chromatin, consist of a 147 bp DNA fragment wrapped around a protein octamer containing two copies each of histones H2A, H2B, H3, and H4. Histones exhibit a variety of posttranslational modifications (PTMs) that are recognized by multimeric effector proteins and act in concert to control gene expression. Deep work has been made in the last years to highlight the combinations of histone marks that concomitantly occur within a nucleosome, with the new front-line focused to evidence whether a specific modification is present on one or on both residues of each histone pair. Accordingly, growing evidence has accumulated on the existence and function of the so-called nucleosomal “bivalent domains” where activating and repressive PTMs added on different residues coexist at the promoters of developmentally regulated genes in embryonic stem cells that resolve this apparently contradictory pattern upon differentiation. We asked whether asymmetric nucleosomes are present also in somatic cells and pointed our attention on one of the best characterized inhibitory PTMs, trimethylated lysine 9 in histone H3 (H3K9me3).