A Mouse Model of Pseudomonas aeruginosa Pneumonia
- Resource Type
- Authors
- Brian W. LeBlanc; Craig T. Lefort
- Source
- Methods in Molecular Biology ISBN: 9781071614877
- Subject
- 0301 basic medicine
Lung
Innate immune system
business.industry
Respiratory infection
Inflammation
medicine.disease
Sepsis
03 medical and health sciences
Pneumonia
030104 developmental biology
0302 clinical medicine
medicine.anatomical_structure
Immunology
Leukocyte Trafficking
medicine
030212 general & internal medicine
medicine.symptom
Bolus (digestion)
business
- Language
The rapid innate immune response to respiratory infection is essential to prevent the systemic dissemination of pathogens. This chapter outlines an experimental mouse model of respiratory infection by gram-negative Pseudomonas aeruginosa and analyses of leukocyte trafficking in the lungs. The reader will learn two methods to induce respiratory infection in mice that differ in whether the initial bolus is targeted within a specific lobe of the lung. We then describe a technique based on tissue digestion and flow cytometry that allows the investigator to distinguish leukocytes within different compartments of the lung, and discuss the advantages and limitations to such an approach.