Figure S2. Efficient and selective inactivation of MFP2 in microglia in Cx3cr1-Mfp2−/− mice. (A) Microglia were isolated from 11-month-old control and Cx3cr1-Mfp2−/− mice, and microglia purity was confirmed by the high expression of microglial markers (Tmem119 and P2ry12) in the positive (microglia) versus the negative fraction (neurons, astrocytes, and oligodendrocytes). Transcript expression of Mfp2 was determined in control and Cx3cr1-Mfp2−/− in both positive and negative fraction. Representative experiment out of two with similar results. (B) MFP2 activity in brain homogenates of control and Cx3cr1-Mfp2−/− mice. n = 2–3 mice/group. Mean ± SD is shown. (PPTX 46 kb)