Identification and Differentiation of Coagulase-Negative Staphylococcus aureus by Polymerase Chain Reaction
- Resource Type
- Authors
- Stephen P. Oliver; Karl R. Matthews; Barbara E. Gillespie; J. Roberson; D. A. Luther
- Source
- Journal of food protection. 60(6)
- Subject
- Micrococcaceae
Biology
medicine.disease_cause
biology.organism_classification
Microbiology
Virology
law.invention
Coagulase test
DNA profiling
Staphylococcus aureus
law
medicine
Effective treatment
Coagulase
Bacteria
Polymerase chain reaction
Food Science
- Language
- ISSN
- 1944-9097
Staphylococcus aureus is an etiological agent of a wide variety of human and animal infections. The majority of S. aureus are coagulase-positive; however, some may be atypical in that they do not produce coagulase. Incorrect identification of an isolate can impact implementation of effective treatment and/or control measures. In this study, polymerase chain reaction-based DNA fingerprinting was used to differentiate coagulase-positive Staphylococcus aureus (CPSA) from coagulase-negative Staphylococcus aureus (CNSA). A total of 29 CNSA and 50 CPSA were evaluated. PCR-based DNA fingerprinting differentiated CNSA from CPSA on the basis of visible observation and densitometric evaluation. The method is rapid and accurate, eliminating variability associated with conventional techniques.