Additional file 3: Figure S2. Sensitivity of EDITS primers. A Nested PCR primers nF6026 and nR6773 were evaluated using a serial dilution of DNA from the HIV-1 molecular clone pNL4-3 (0% to 100%) in a background of DNA from the non-HIV plasmid pUC19, at a final concentration of 0.1 ng/ml. DNA mixtures were amplified using Standard and Real-time PCR. Amplicons from the standard PCR were also deep sequenced and vpu/env mapped reads quantified using the DEEPGEN��� Software Tool Suite. Mean mapped reads and standard deviation are depicted. B ACH-2, a cell line latently infected with a copy of HIV-1 per cell, and MT-4, an HIV-negative human T cell line, were quantified and serial dilutions used to prepare seven mixtures containing 0 to 1000 CH-2 cells in a background of one million MT-4cells. Cell mixtures were ctivated with 100 ��g/ml of Concanavalin A and cell-associated spliced HIV-1 RNA quantified using EDITS as described in ���Methods��� section. Mean mapped reads and standard deviation are depicted.