The isolation and identification of sixteen compounds extracted from the aerial parts of A. fruticulosa have been reported in the present study, including eight flavonoids (1, 2, 3, 4, 5, 6, 7 and 8), three terpenoids (9, 10 and 11), two sterols (12 and 13), one lignan (14), one fatty acid (15) and one fatty acid ethyl ester (16), wherein six compounds (2, 3, 4, 5, 10 and 14) have been isolated from A. fruticulosa for the first time. Furthermore, among the identified compounds, three compounds (6, 7 and 11) have also been reported for the first time in the genus Ajanin and three compounds (8, 15 and 16) have not been isolated and reported from other plants of the family Asteraceae. In addition, the chemotaxonomic significance of these compounds was discussed.