Combination of all-trans retinoic acid and lithium chloride surmounts a retinoid differentiation block induced by expression of Scl and Rbtn2 transcription factors in myeloid leukemia cells
- Resource Type
- Authors
- Anna M. Rice; Jianming Li; Alan C. Sartorelli
- Source
- Leukemia Research. 28:399-403
- Subject
- Acute promyelocytic leukemia
Cancer Research
medicine.medical_specialty
medicine.drug_class
HL60
Cellular differentiation
Blotting, Western
Retinoic acid
Antineoplastic Agents
HL-60 Cells
Tretinoin
Transfection
chemistry.chemical_compound
Adjuvants, Immunologic
Proto-Oncogene Proteins
hemic and lymphatic diseases
Internal medicine
Metalloproteins
Basic Helix-Loop-Helix Transcription Factors
medicine
Animals
Humans
Retinoid
neoplasms
Transcription factor
T-Cell Acute Lymphocytic Leukemia Protein 1
Adaptor Proteins, Signal Transducing
Leukemia
organic chemicals
fungi
Myeloid leukemia
Cell Differentiation
Drug Synergism
Hematology
LIM Domain Proteins
medicine.disease
biological factors
DNA-Binding Proteins
Endocrinology
Oncology
chemistry
Cancer research
Lithium Chloride
Transcription Factors
- Language
- ISSN
- 0145-2126
We have previously shown that forced expression of the transcription factor Scl in WEHI-3B D(+) cells prevents ATRA-induced cell differentiation. We now find that the overexpression of Rbtn2 also interferes with induction of differentiation by ATRA. Addition of LiCl to ATRA treatment restored the capacity of both Scl- and Rbtn2-expressing cells to respond to the retinoid in a synergistic manner. Similar results were obtained with Scl-transfected HL60 cells where its expression diminished responsiveness to ATRA. These findings suggest that if Scl and/or Rbtn2 are involved in the non-responsiveness of AML patients to ATRA-induced differentiation, addition of LiCl may reverse insensitivity.