Studies have reported that cannabis consumption causes acute effects on the brain and behavior, with anxiety effects being some of the most studied. It has also been shown that chronic cannabis use develops tolerance to some of the effects of the drug (i.e., when the effect of a dose of a given drug decreases over the course of repeated administrations). However, no one has yet, to our knowledge, evaluated whether the behavioral tolerance to cannabis is context-specific, as a perspective based on Pavlovian conditioning would suggest, even though this approach has proven to be a robust explanation of tolerance to other drugs (i.e., ethanol). This project aims to examine the acute effects, chronic tolerance, and the contextual specificity of tolerance to the effects of cannabis on the anxiety of adult rats. For this purpose, we will use 24 Sprague-Dawley adult rats, which will be separated into 3 experimental groups (n = 8), divided according to drug concentration exposure (cannabis extract with either 0, 75, or 150 mg/ml of THC). Training and test trials will be carried out in an open field 5 min before and 5 min after the vaporized administration of the drug, measuring velocity, distance traveled, rearing, grooming, time spent in the center, among other variables associated with anxiety. Contexts and sex will be counterbalanced between groups. The experiment has three specific objectives according to each variable of interest: Specific Objective 1 (SO1): To evaluate and compare between groups the acute effects of cannabis on the anxiety of adult rats. Specific Objective 2 (SO2): To evaluate and compare between groups the development of chronic tolerance to the effects of cannabis on the anxiety of adult rats. Specific Objective 3 (SO3): To evaluate and compare between groups the context specificity of tolerance to the effects of cannabis on the anxiety of adult rats. Acknowledgment: We are grateful to the National Agency for Research and Development of Chile (ANID-Chile) for supporting the work of the Research Group in Experimental Psychology and Psychopathology, Department of Psychology, Faculty of Social Sciences, University of Chile. This study is funded by project ANID-Fondecyt 1191619 granted to Mario Laborda.