The production of neuraminidase (sialidase, EC 3.2.1.18) by Clostridium chauvoei (Jakari strain) was investigated and a novel neuraminidase was detected in the bacterium in vitro using a fluorescent substrate. Neuraminidase activity was reproducible and had a linear relationship with the amount of bacteria at lower bacterial concentrations. The relationship was no longer linear at higher bacterial concentrations (3.33×107 to 4.44×107 cfu/ml). The possible role of this enzyme in the pathogenesis of blackleg disease is discussed. It is concluded that the enzyme could be playing the role of spreading the disease in the tissues of infected ruminants. Therefore, the use of neuraminidase inhibitors to manage the disease clinically should be thoroughly investigated.
Dokazana je proizvodnja neuraminidaze (sialidaze, EC 3.2.1.18) bakterije Clostridium chauvoei soja Jakari in vitro pomoću fluorescentnog supstrata. Aktivnost neuraminidaze bila je reproducibilna uz linearni odnos količine bakterija pri nižim bakterijskim koncentracijama. Taj omjer nije bio linearan pri višim bakterijskim koncentracijama (3,33 x 107 do 4,44 x 107 cfu/ml). Raspravlja se o mogućoj ulozi ovog enzima u patogenezi šuštavca. Zaključeno je da bi enzim mogao imati ulogu u širenju bolesti kroz tkiva inficiranih preživača. Uporaba inhibitora neuraminidaze u kontroli bolesti trebala bi biti potpunije istražena.