Increased striatal VMAT2 binding in mice after chronic administration of methcathinone and manganese
- Resource Type
- Authors
- Sulev Kõks; Anniina Snellman; Jaak Nairismägi; Andres Asser; Mall Eltermaa; Eveliina Arponen; Merja Haaparanta-Solin; Martin Sauk; Ursel Soomets; Jonas Bergquist; Hanna Lindmäe; Juha O. Rinne; Piret Piip; Tove J. Grönroos; Pille Taba
- Source
- BRAIN RESEARCH
- Subject
- 0301 basic medicine
Male
Time Factors
medicine.medical_treatment
Tetrabenazine
Pharmacology
Methcathinone
Dihydrotetrabenazine
chemistry.chemical_compound
Random Allocation
0302 clinical medicine
Extrapyramidal symptoms
Manganism
Medicine
Carbon Radioisotopes
Saline
Gait
Propiophenones
General Neuroscience
Dopaminergic
Biomechanical Phenomena
medicine.symptom
Injections, Intraperitoneal
medicine.drug
Substance-Related Disorders
Motor Activity
ta3111
ta3112
03 medical and health sciences
Animals
Molecular Biology
Manganese
Psychotropic Drugs
business.industry
Illicit Drugs
medicine.disease
Corpus Striatum
Vesicular monoamine transporter
Mice, Inbred C57BL
Disease Models, Animal
030104 developmental biology
chemistry
Positron-Emission Tomography
Vesicular Monoamine Transport Proteins
Autoradiography
Central Nervous System Stimulants
Neurology (clinical)
Radiopharmaceuticals
business
030217 neurology & neurosurgery
Ex vivo
Developmental Biology
- Language
- ISSN
- 1872-6240
Intravenous use of a psychostimulant drug containing methcathinone (ephedrone) and manganese causes an irreversible extrapyramidal syndrome in drug abusers. We aimed to reproduce the syndrome in mice to evaluate dopaminergic damage. C57/B6 mice were intraperitoneally injected once a day with the study drug or saline for a period of 27 weeks. Motor activity was recorded in an automated motility-box. After 13 and 27 weeks of treatment, ex vivo digital autoradiography was performed using [11C]dihydrotetrabenazine ([11C]DTBZ). After 27 weeks of treatment [11C]DTBZ autoradiography demonstrated a significant increase in the striatum-to-cerebellum binding ratio compared with saline treated controls. At the same time point, there was no evident change in motor activity. Increased [11C]DTBZ binding may indicate vesicular monoamine transporter type 2 (VMAT2) function is altered. The lack of extrapyramidal symptoms in animals could be attributed to low dosing regimen or high metabolic rate.