Rapid molecular detection and genotyping of West Nile Virus lineages 1 and 2 by real time PCR and melting curve analysis
- Resource Type
- Authors
- Riccardo De Santis; Roberto Bei; Florigio Lista; Giovanni Faggioni; Federica Monaco; Massimo Fantini; Andrea Polci; Giovanni Savini; Alice Pomponi
- Source
- Journal of virological methods. 207
- Subject
- Melting curve analysis
Genotyping Techniques
West Nile virus
viruses
Lineage (evolution)
Genome, Viral
medicine.disease_cause
Real-Time Polymerase Chain Reaction
Genome
Virus genotyping
WNV
Virology
medicine
Consensus sequence
Transition Temperature
Genotyping
Molecular detection
DNA Primers
Settore MED/04 - Patologia Generale
biology
Flavivirus
virus diseases
biology.organism_classification
United States
Europe
Real-time polymerase chain reaction
RNA, Viral
Oligonucleotide Probes
West Nile Fever
- Language
- ISSN
- 1879-0984
Following its spread in the USA, West Nile Virus (WNV) has reemerged in the Mediterranean basin with a renewed pathogenicity. The introduction of WNV lineage 2 in Europe and its co-circulation with lineage 1 has resulted in a continuously changing epidemiological scenario, highlighting the importance of differential detection of the two lineages. The paper describes a new real-time PCR method for the detection and genotyping of the two main lineages of WNV. The method requires a single pair of primers and probes and is based on the analysis of highly conserved consensus sequences detected in the 5′ terminus of the viral genome.