How to improve the sensitivity of coplanar electrodes and micro channel design in electrical impedance flow cytometry: a study
- Resource Type
- Authors
- Jonathan Cottet; Marie Frénéa-Robin; François Buret; Philippe Renaud; Alexandre Kehren; Harald van Lintel
- Source
- Microfluidics and Nanofluidics
Microfluidics and Nanofluidics, Springer Verlag, 2019, 23 (1), ⟨10.1007/s10404-018-2178-6⟩
- Subject
- Materials science
Acoustics
Impedance spectroscopy
02 engineering and technology
01 natural sciences
law.invention
Electrode design
[SPI]Engineering Sciences [physics]
Lab-on-a-Chip
law
Materials Chemistry
Sensitivity (control systems)
Flow cytometry
Electrical impedance
Microchannel
010401 analytical chemistry
Dielectrophoresis
Lab-on-a-chip
021001 nanoscience & nanotechnology
Condensed Matter Physics
Finite element method
0104 chemical sciences
Electronic, Optical and Magnetic Materials
Electrode
0210 nano-technology
Material properties
- Language
- English
- ISSN
- 1613-4982
1613-4990
International audience; This paper describes a comprehensive analysis of the geometrical parameters influencing the sensitivity of a coplanar electrode layout for electrical impedance flow cytometry. The designs presented in this work have been simulated, fabricated, and tested. 3D finite element method was applied to simulate and improve the sensitivity of the coplanar designs for two spacings between electrodes. The proposed model uses conditional expressions to define spatially dependent material properties. The vertical and lateral sensitivities were evaluated for all the designs. The experimental results obtained with polystyrene beads show good agreement with the simulations. Precentering particles with dielectrophoresis allowed to control their position in the microchannel. The optimized designs are envisioned to be used for sizing and characterizing particles from single cells to cell aggregates.