Interactions between SAM and the 5′ UTR mRNA of the sam1 gene regulate translation in S. pombe
- Resource Type
- Authors
- Wenxia Sun; Alastair I.H. Murchie; Xuhui Zhang; Dongrong Chen
- Source
- RNA
- Subject
- Untranslated region
S-Adenosylmethionine
Five prime untranslated region
Biology
Ligands
Article
03 medical and health sciences
Genes, Reporter
Gene Expression Regulation, Fungal
Schizosaccharomyces
RNA, Messenger
Molecular Biology
Gene
030304 developmental biology
0303 health sciences
Messenger RNA
030302 biochemistry & molecular biology
RNA
Translation (biology)
RNA, Fungal
Methionine Adenosyltransferase
biology.organism_classification
Protein tertiary structure
Cell biology
Protein Biosynthesis
Schizosaccharomyces pombe
5' Untranslated Regions
- Language
- English
The 5′ untranslated region (5′ UTR) of eukaryotic mRNA plays an important role in translation. Here we report the function of the 5′ UTR mRNA of S-adenosylmethionine synthetase (sam1) in translational modulation in the presence of SAM in fission yeast Schizosaccharomyces pombe. Reporter assays, binding and chemical probing experiments, and mutational analysis show that the 5′ UTR mRNA of sam1 binds to SAM to effect translation. Translational modulation is dependent on a tertiary structure transition in the RNA upon SAM binding. The characterization of such an RNA that is directly associated with an essential metabolic process in eukaryotes provides additional evidence that ligand binding by RNAs plays an important role in eukaryotic gene regulation.