Alteration of protein kinase C conformation in red blood cells: A potential marker for Alzheimer's disease but not for Parkinson's disease
- Resource Type
- Authors
- Christian Marescaux; Jean de Barry; François Sellal; Jean-Marie Danion; Agnes Janoshazi; Jean Marie Warter
- Source
- Neurobiology of Aging. 27:245-251
- Subject
- Male
Aging
Pathology
medicine.medical_specialty
Erythrocytes
Indoles
Parkinson's disease
Protein Conformation
Blood cell
Central nervous system disease
Degenerative disease
Alzheimer Disease
Internal medicine
Phorbol Esters
medicine
Humans
Dementia
Enzyme Inhibitors
Protein Kinase C
Protein kinase C
Aged
Analysis of Variance
business.industry
Spectrum Analysis
General Neuroscience
Age Factors
Parkinson Disease
Fluoresceins
Staurosporine
medicine.disease
Red blood cell
medicine.anatomical_structure
Endocrinology
Female
Neurology (clinical)
Geriatrics and Gerontology
Alzheimer's disease
business
Biomarkers
Developmental Biology
- Language
- ISSN
- 0197-4580
There is a growing evidence of early changes of blood cells in Alzheimer's disease (AD). We have developed an original novel method for quantifying the alteration of protein kinase C (PKC) by its fluorescence spectrum: by using Fim-1, a specific fluorescent probe made for protein kinase C that detects the conformational changes of this. We show that the PKC conformation is altered in red blood cells (RBC) from AD patients as compared to RBC from healthy controls. This alteration is independent of the patient's age and of the stage of the disease. It is not observed in the RBC of non-demented patients suffering from Parkinson's disease (PD). If PKC alteration is proven to be specific to AD as compared with other dementia, this method could be for a simple, low cost screening test among patients suspected of having AD and may have a strong predictive value.