Enhanced basal autophagy supports ameloblastoma-derived cell survival and reactivation
- Resource Type
- Authors
- Olajumoke Ajibola Effiom; Sunday O. Akintoye; Kathleen Boesze-Battaglia; Adetokunbo Babajide Olawuyi; G T Arotiba; Rachel C. Sharp; Anuradha Dhingra; Onatolu Odukoya
- Source
- Archives of Oral Biology. 98:61-67
- Subject
- 0301 basic medicine
Epithelial-Mesenchymal Transition
Cell Survival
Odontogenic Tumors
Biology
Article
Epithelium
Malignant transformation
Ameloblastoma
Mice
03 medical and health sciences
Basal (phylogenetics)
0302 clinical medicine
Sequestosome-1 Protein
Ameloblasts
Autophagy
medicine
Animals
Humans
Clonogenic assay
General Dentistry
Mesenchymal stem cell
Intracellular Signaling Peptides and Proteins
Mesenchymal Stem Cells
030206 dentistry
Cell Biology
General Medicine
medicine.disease
Xenograft Model Antitumor Assays
Adaptor Proteins, Vesicular Transport
Disease Models, Animal
030104 developmental biology
Otorhinolaryngology
Cancer research
Heterografts
Female
Neoplasm Recurrence, Local
Stem cell
Carrier Proteins
Microtubule-Associated Proteins
Solid/Multicystic Ameloblastoma
- Language
- ISSN
- 0003-9969
OBJECTIVES: Ameloblastoma is an aggressive odontogenic jaw neoplasm. Its unlimited growth confers high potential for malignant transformation and recurrence. It is unclear why ameloblastoma is highly recurrent despite surgical resection with a wide margin of normal tissue. While canonical autophagy can be used to degrade and eliminate damaged cellular components, it is also a protective mechanism that provides energy and vital metabolites for cell survival. We used ameloblastoma-derived cells to test the hypothesis that autophagic processes play a role in survival and reactivation of ameloblastoma. METHODS: Primary epithelial (EP-AMCs) and mesenchymal (MS-AMCs) ameloblastoma-derived cells were established from tissue samples of solid multicystic ameloblastoma. Clonogenic capacity and basal autophagic capacity were assessed in ameloblastoma-derived cells relative to human odontoma-derived cells (HODCs) and maxilla-mesenchymal stem cells (MX-MSCs). Ability of ameloblastoma-derived cells to survive and form new ameloblastoma was assessed in mouse tumor xenografts. RESULTS: EP-AMCs were highly clonogenic (p < 0.0001) and demonstrated enhanced basal levels of autophagic proteins microtubule-associated protein 1-light chain 3 (LC3) (p