In-Silico Analysis of Chromatin Modifiers and Profiling of Histone Deacetylases (HDAC's) in Human Oral Cancer
- Resource Type
- Authors
- Sanjay Gupta; Abhiram Natu; Anand K. Sajnani; Mudasir Rashid; Poonam Gera; Sanket Shah
- Source
- Chonnam Medical Journal
- Subject
- biology
business.industry
Histone deacetylase 2
General Engineering
Cancer
RNA
Oral Squamous Cell Carcinoma
Histone Acetylases
medicine.disease
Histone Deacetylases
Chromatin
stomatognathic diseases
Histone
medicine.anatomical_structure
Tongue
biology.protein
Cancer research
Medicine
Original Article
Epigenetics
RNA extraction
business
- Language
- ISSN
- 2233-7393
2233-7385
Histone modifications have been demonstrated to play a significant role in oral squamous cell carcinoma (OSCC) epigenetic regulation. An in-silico analysis of The Cancer Genome Atlas (TCGA) of various histone acetyl transferases (HATs) and histone deacetylases (HDACs) suggested that HATs do not differ between normal and tumor samples whereas HDAC2 and HDAC1 change maximally and marginally respectively between normal and tumor patients with no change being noted in HDAC6 expression. Hence, this investigation was carried out to validate the expression states of HDAC 1, 2 and 6 mRNAs in buccal mucosa and tongue SCC samples in an Indian cohort. Buccal mucosa and tongue squamous cell carcinoma tissues with intact histopathology were processed for RNA isolation followed by cDNA synthesis which was then subjected to q-PCR for HDACs. The average RNA yield of the tongue tissue sample was ∼2 µg/mg of tissue and the A260/280 ratios were between 2.03 and 2.06. The average RNA yield of buccal mucosa tissue sample was ∼1 µg/mg of tissue and the A260/280 ratio were between 2.00 and 2.08. We have demonstrated that HDAC2 was overexpressed in tongue and buccal mucosa samples. Over-expression of HDAC2 imply potential use of HDACi along with standard chemotherapeutic drug in oral cancer treatment.