LOrnithine could serve as an intermediate in the biobased production of 1,4diaminobutane from Larginine. Using the concept of biorefinery, Larginine could become widely available from biomass waste streams viathe nitrogen storage polypeptide cyanophycin. Selective hydrolysis of Larginine to Lornithine is difficult to perform chemically, therefore the stabilization and immobilization of Bacillus subtilisarginase EC3.5.3.1 was studied in a continuously stirred membrane reactor system. Initial pH of the substrate solution, addition of Laspartic acid and reducing agents all appeared to have an effect on the operational stability of B. subtilisarginase. A remarkably good operational stability total turnover number, TTN1.13⋅108 at the pH of arginine free base pH11.0 was observed, which was further improved with the addition of sodium dithionite to the substrate solution TTN>1⋅109. B. subtilisarginase was successfully immobilized on three commercially available epoxyactivated supports. Immobilization on Sepabeads ECEP was most promising, resulting in a recovered activity of 75% and enhanced thermostability. In conclusion, the stabilization and immobilization of B. subtilisarginase has opened up possibilities for its application in the biobased production of nitrogencontaining chemicals as an alternative to the petrochemical production.