Three-dimensional dynamics optical coherence tomography for tumor spheroid evaluation
- Resource Type
- Article
- Authors
- El-Sadek, Ibrahim Abd; Miyazawa, Arata; Shen, Larina Tzu-Wei; Makita, Shuichi; Mukherjee, Pradipta; Lichtenegger, Antonia; Matsusaka, Satoshi; Yasuno, Yoshiaki
- Source
- Biomedical Optics Express; November 2021, Vol. 12 Issue: 11 p6844-6863, 20p
- Subject
- Language
- ISSN
- 21567085
We present a completely label-free three-dimensional (3D) optical coherence tomography (OCT)-based tissue dynamics imaging method for visualization and quantification of the metabolic and necrotic activities of tumor spheroid. Our method is based on a custom 3D scanning protocol that is designed to capture volumetric tissue dynamics tomography images only in a few tens of seconds. The method was applied to the evaluation of a tumor spheroid. The time-course viability alteration and anti-cancer drug response of the spheroid were visualized qualitatively and analyzed quantitatively. The similarity between the OCT-based dynamics images and fluorescence microscope images was also demonstrated.