A comparative assessment of purification techniques for mesophyll protoplasts of Brassica napus L.
- Resource Type
- Article
- Authors
- Millam, Stephen; Burns, Alan T. H.; Hocking, Trevor J.
- Source
- Plant Cell, Tissue and Organ Culture; January 1991, Vol. 24 Issue: 1 p43-47, 5p
- Subject
- Language
- ISSN
- 01676857; 15735044
The effects of three different general purification protocols have been assessed quantitatively using mesophyll protoplasts of Brassica napus. Within the initial sample two distinct sub-populations were determined. The methods used influenced the ratio of the vacuolated to chloroplastic type protoplast sub-populations. Overall recovery rates of the initial sample varied according to the method used from 38% to 27%, but the relative recovery of the sub-populations varied considerably with a purified ratio of between 1.0:0.78 to 1.0:7.0. Size distribution profiles of the initial and purified populations are also presented.