Purpose: We present a video demonstration of the endoscopic management of a Gartner’s duct cyst presenting as hydrocolpos. Methods: A 3-year-old girl presented with abdominal pain and increased frequency of micturition for several months, but preserved urinary continence, and no UTIs. Ultrasound showed hydrocolpos and normal simplex kidneys. EUA revealed normal external genitalia. Vaginoscopy demonstrated a cystic structure inside the vagina arising from the anterior wall, and a normal cervix. A normal bladder with two orthotopic ureters was seen, and a bulge was noted in the trigonal area. Results: A 11Fr resectoscope with a cold knife was used to incise the cyst transvaginaly. The cyst cavity was entered which drained clear fluid and no other lesions or openings were noted. Literature review suggested the diagnosis of Gartner’s duct cyst. Conclusions: A simple endoscopic incision of Gartner’s duct cyst can be an effective and safe treatment modality in select cases, with no urological abnormalities. A good understanding of the anatomy and exclusion of concomitant anomalies is mandatory to achieve the correct diagnosis and the best outcome.