A Comparison of Ypet and Firefly Luciferase as Reporter Proteins for High-Throughput Screening
- Resource Type
- Article
- Authors
- YOON, Sei Mee; NAMKUNG, Wan; LEE, Jinu
- Source
- Bioscience, Biotechnology, and Biochemistry; November 2013, Vol. 77 Issue: 11 p2328-2330, 3p
- Subject
- Language
- ISSN
- 09168451; 13476947
When Ypet was used as a reporter protein for high-throughput screening (HTS), it showed peak fold induction and a dynamic range similar to those for firefly luciferase. We also determined that conducting a reading immediately after media aspiration was the best method for HTS. We conclude that Ypet can serve as a substitute for luciferase as a reporter protein in HTS assays.