Polycystic ovary syndrome (PCOS) is an endocrine disorder, which affects 4-7% women in the reproductive age worldwide. The women with PCOS present hyperandrogenism, oligomenorrhea or polycystic ovaries. It is well recognized as the pathophysiology of the following disorders, including hirsutism, obesity, insulin resistance and metabolic disturbance. Although the etiology of PCOS is still unknown, hyperandrogenism is now considered as the key role of the syndrome. Recently, several animal models have been established and explored to understand the ovarian dysfunction and metabolic disorders. However, the effects of androgen on the glucocorticoid production in androgenized PCOS model has not been well investigated. In the present study, the peripubertal androgenized female rats were conducted. Four-wk old female rats were treated with testosterone propionate (TP) or 5alpha-dihydrotestosterone (DHT) subcutaneously for 10 weeks. Both TP and DHT rats showed acyclic, loss adrenal weights after peripubertal androgenization and the releases of both adrenocorticotropic hormone (ACTH) and corticosterone were reduced. Studies of the androgen effects on the regulation of hypothalamic-pituitary-adrenal axis via negative feedback showed upregulation of glucocorticoid receptor (GR) protein expression in anterior pituitary (AP) of TP and DHT rats. Furthermore, in vitro DHT treatment elevated GR expression in AP. These results suggested that by increasing the sensitivity of GR-mediated negative feedback regulation at AP level, peripubertal androgenization in female rats reduced ACTH secretion and corticosterone production. This study may provide more information about the role of androgen in regulating glucocorticoid production in female and PCOS rats.(platform)