β-Glucosidase belongs to the glycoside hydrolase I family, which is widely present in multiple species and responds to various biotic and abiotic stresses. In rice, whether β-glucosidase is involved in the interaction between plants and microorganisms is not clear. In this study, we found that the expression of several genes encoding β-glucosidases, including OsBGLU19and OsBGLU23, were upregulated after inoculation with Xanthomonas oryzaepv. oryzicola(Xoc) and downregulated after inoculation with X. oryzaepv. oryzae(Xoo). The respective insertion mutants of OsBGLU19and OsBGLU23, bglu19and bglu23, were more susceptible to Xocinfection. The expression of OsAOS2, a key gene in the jasmonic acid signal pathway, was dramatically downregulated after inoculation with Xocin the bglu19and bglu23mutants. Simultaneously, the expression of downstream disease resistance-related genes, such as OsPR1a, OsPR5and a key transcription factors OsWRKY72were obviously downregulated. The resistance mediated by OsBGLU19and OsBGLU23to bacterial leaf streak is related to disease resistance-related genes above mentioned.