Protocol for changing gene expression in 3T3-L1 (pre)adipocytes using siRNA-mediated knockdown
- Resource Type
- Article
- Authors
- Strnadová, Martina; Thor, Doreen; Kaczmarek, Isabell
- Source
- STAR Protocols; 20240101, Issue: Preprints
- Subject
- Language
- ISSN
- 26661667
3T3-L1 is a model cell line which can be differentiated from preadipocytes into mature adipocytes. Here, we present a protocol for changing gene expression in 3T3-L1 (pre)adipocytes using siRNA-mediated knockdown. We describe steps to perform the knockdown of a certain gene prior to differentiation (day -4) to analyze the impact on adipogenesis. We then detail procedures for knockdown on differentiation day 8 to study the role of a certain gene in mature adipocyte function.