The global tomato cultivation faces serious threats from the whitefly Bemisia tabaci (Gennadius) and the invasive tomato leafminer Tuta absoluta (Meyrick). Identifying new resistant sources and exploring resistance mechanisms is crucial for long-term management of these rapidly spreading insects. This study evaluated antixenosis and antibiosis resistance in wild tomato accessions of Solanum cheesmaniae and Solanum galapagense through trichome characteristics and various assays, revealing resistance to B. tabaci (Asia II 7 biotype) and T. absoluta. Wild accessions resulted in reduced oviposition, increased adult mortality of whiteflies, fewer whitefly developmental stages, disrupted leafminer developmental stages, and reduced adult emergence. Moreover, high densities of type-IV glandular trichomes in wild accessions correlated negatively with the resistance parameters of both insects, influencing their preference and performance. Variations in resistance parameters were identified between wild and cultivated accessions, with S. galapagense (VI063117-10) demonstrating superior resistance. This study emphasizes the significance of understanding resistance traits in wild accessions to manage pest insects with diverse feeding behaviors and utilize them in developing multiple insect-resistant tomato varieties.