AbstractIn this present study, we describe the chemical composition, larvicidal and molluscicidal activity of essential oil distilled from Alpinia zerumbetleaves against third-stage larvae of Ae.aegyptiand the snail Biomphalaria glabrata, respectively. For this, we distilled the oil by hydrodistillation, identified its components by gas chromatography coupled to mass spectrometry and gas chromatography with flame ionization detection and calculated the lethal concentration of larvicidal and molluscicidal activity using the probit method. From these analyses, we showed that the oil is predominantly composed of monoterpenes, in which p-cymene (34.12%), 1,8-cineole (25.67%) and terpinen-4-ol (21.01%) stood out. In addition, we also proved that the oil has larvicidal and molluscicidal activity with lethal concentrations respectively of 43.74 and 36.52 μg/mL. Therefore, we conclude that the oil is active and has the potential to replace synthetic larvicides and molluscicides.