Saccharum spontaneum, S. robustumand S. officinarumare the founding polyploid genomes of the modern varieties of sugarcane (Saccharumspp.). The crop is one of the world's major sugar and biomass-producing crops, potential sources of renewable energy. Understanding the genomic relationships among the founding genomes is crucial if we are to develop disease/drought-resistant varieties to counter the threat posed by climate change. Saccharumcytogenetics is a growing field, and significant progress has been achieved particularly in S. spontaneum(x= 8, 2n= 5 – 16x= 40 – 128, commonly2n= 8x= 64) and S. officinarum(x= 10, 2n= 8x= 80). However, only a few studies on S. robustum(x= 10, 2n= 6 − 20x= 60 − 200) have been conducted, which is thought to be the wild ancestor of S. officinarum, a legitimate species, commonly known as noble cane. Herein, using fluorescent in situ hybridization with centromeric probes we found clear evidence that S. robustummeiosis is regular (2n= 100), exhibiting 50 bivalents, with no univalents or multivalent associations. In addition, using genomic in situ hybridization, we found a close relationship between S. robustumand S. officinarumand divergence from S. spontaneum.