Differentiation of myeloid cells is accompanied by increased levels of pp60c-src protein and kinase activity.
- Resource Type
- Article
- Authors
- Gee, C E; Griffin, J; Sastre, L; Miller, L J; Springer, T A; Piwnica-Worms, H; Roberts, T M
- Source
- Proceedings of the National Academy of Sciences of the United States of America; July 1986, Vol. 83 Issue: 14 p5131-5135, 5p
- Subject
- Language
- ISSN
- 00278424; 10916490
We have detected a significant increase in the levels of pp60c-src kinase activity associated with the differentiation of myeloid cell lines HL-60 and U-937. The induction of pp60c-src kinase activity becomes apparent approximately 14 hr after the addition of phorbol 12-myristate 13-acetate and increases 20-fold by 72 hr. The enhanced kinase activity can be accounted for by elevated levels of c-src protein in the differentiated cells. When nonleukemic bone marrow cells were examined, myeloid progenitor cells exhibited a low level of pp60c-src kinase activity. As these cells are allowed to differentiate in culture, the resulting adherent monocytes are as high in pp60c-src kinase activity as HL-60 cells induced to differentiate into monocytes. A strong correlation is found between the levels of pp60c-src kinase activity and the degree of monocytic differentiation of the cells from patients with acute myeloid leukemia. Our findings suggest that the activation of pp60c-src kinase activity is a normal physiological event associated with myeloid differentiation.