Integrative promoter cloning plasmid vectors for Rhizobium meliloti
- Resource Type
- Article
- Authors
- Élő, P; Semsey, Sz; Kereszt, A; Nagy, T; Papp, P; Orosz, L
- Source
- FEMS Microbiology Letters; February 1998, Vol. 159 Issue: 1 p7-13, 7p
- Subject
- Language
- ISSN
- 03781097; 15746968
A set of integrative ‘promoter probe' plasmids were constructed for both translational and transcriptional fusions. The vectors are based on the broad host range, low copy number plasmid pRK290 (IncP1) in which the attachment site of Rhizobiumphage 16‐3and the lacZgene of Escherichia coliwere combined. The vectors integrate into the chromosome of Rhizobium meliloti, providing also the advantages of the single copy promoter probe cassettes. Thus they fulfil the prerequisite of the systems used for investigating gene regulation. The plasmids were applied for the study of the transcription regulation of the 16‐3phage. Their versatile use is also demonstrated.