Chromatographic resolution of a series of derivatized amino acids was attempted on a column packed with an ion-exchange adduct of ?-[Ru(phen)3]2+(phen = 1,10-phenanthroline) and synthetic hectorite. An amino acid was modified to N-3, 5-dinitrobenzoyl amino acid methyl ester (denoted by DNB-aa-me). For aa = Ala, Phe, Leu, Ile, Ser, Val, Thr, Tyr, Asp and Glu, racemic DNB-aa-me was resolved nearly to baseline separation on being eluted with methanol. For aa = Trp and His, racemic DNB-aa-me was resolved partially. No resolution was achieved for Pro and Lys. The mechanism of chiral discrimination was investigated by means of solid-state vibrational circular dichroism spectroscopy.Chromatographic resolution of a series of derivatized amino acids was achieved on a column packed with an ion-exchange adduct of ?-[Ru(phen)3]2+and synthetic hectorite. The association structure is predicted by means of vibrational circular dichroism spectroscopy. These features may make the present column a unique member of columns in the field of d-amino acid analysis.