The aerial parts of Artemisia frigidaWilld. are used to treat joint swelling, renal heat, abnormal menstruation, and sore carbuncle. The anti-inflammatory effects of A. frigidahave been well-known in folk medicine, suggesting that components extracted from A. frigidacould potentially treat inflammatory disease. With the aim of discovering bioactive compounds, in this study, we extracted total flavonoids from the aerial parts of A. frigidaand investigated their anti-inflammatory effects against inflammation induced by carrageenan and egg albumin in rats. At the doses studied, total flavonoids (100 mg/kg, 200 mg/kg, and 400 mg/kg) and some isolated compounds (30 mg/kg) showed significant and dose-dependent anti-inflammatory effects. According to the high-performance liquid chromatography analysis of the total flavonoids from A. frigida, there are five major compounds, namely, 5-hydroxy-3′,4′-dimethoxy-7-O-β-d-glucuronide (F1), 5-hydroxy-3′,4′,5′-trimethoxy-7-O-β-d-glucuronide (F2), 5,7,3′-trihydroxy-6,4′-dimethoxyflavone (F3), 5,3′-dihydroxy-6,7,4′-trimethoxyflavone (F4), and 5,3′-dihydroxy-3,6,7,4′-tetramethoxyflavone (F5), which may explain the anti-inflammatory activity.