Rhabdomyosarcoma is the most common pediatric soft tissue malignancy. Two major subtypes, alveolar rhabdomyosarcoma and embryonal rhabdomyosarcoma, constitute 20 and 60% of all cases, respectively. Approximately 80% of alveolar rhabdomyosarcoma carry two signature chromosomal translocations, t(2;13)(q35;q14) resulting in PAX3–FOXO1fusion, and t(1;13)(p36;q14) resulting in PAX7–FOXO1fusion. Whether the remaining cases are truly negative for gene fusion has been questioned. We are reporting the case of a 9-month-old girl with a metastatic neck mass diagnosed histologically as solid variant alveolar rhabdomyosarcoma. Chromosome analysis showed a t(8;13;9)(p11.2;q14;9q32) three-way translocation as the sole clonal aberration. Fluorescent in situhybridization (FISH) demonstrated a rearrangement at the FOXO1locus and an amplification of its centromeric region. Single-nucleotide polymorphism-based microarray analysis illustrated a co-amplification of the FOXO1gene at 13q14 and the FGFR1gene at 8p12p11.2, suggesting formation and amplification of a chimerical FOXO1–FGFR1gene. This is the first report to identify a novel fusion partner FGFR1for the known anchor gene FOXO1in alveolar rhabdomyosarcoma.