Staphylococcus aureushas been considered as one of the main pathogens that cause various diseases. Therefore, access to fast and reliable DNA-based methods is crucial for the detection and identification of this bacterium. DNA extraction and purification are fundamental primary steps in almost all molecular biology studies. Therefore, the purpose of this work is utilising Fe3O4/SiO2/TiO2magnetic nanoparticles to extract genomic DNA of Staphylococcus aureus. This paper contains extracting genomic DNA from standard strain of Staphylococcus aureusATCC 25923 using Fe3O4/SiO2/TiO2nanostructures. The quality of extracted DNA was evaluated after electrophoresis on gel agarose, also DNA purity and concentrations were measured by a NanoDrop spectrophotometer. The concentration of genomic DNA extracted by Fe3O4/SiO2magnetic nanoparticles from Staphylococcus aureusstrain ATCC 25923 was 131.635 ng/μL. Also, A260/280 and A260/230 values of mentioned DNA were ranged 1.7 to 1.8 and 2 to 2.2 respectively. The obtained results showed that the DNA extracted by the synthesised magnetic nanoparticles has an acceptable concentration and purity for subsequent molecular biology studies in this bacterium.