Carbon Nanofiber-based Luminol-biotin Probe for Sensitive Chemiluminescence Detection of Protein
- Resource Type
- Article
- Authors
- Baj, Stefan; Krawczyk, Tomasz; Pradel, Natalia; Azam, Golam; Shibata, Takayuki; Dragusha, Shpend; Skutil, Krzysztof; Pawlyta, Mirosława; Kai, Masaaki
- Source
- Analytical Sciences; November 2014, Vol. 30 Issue: 11 p1051-1056, 6p
- Subject
- Language
- ISSN
- 09106340; 13482246
A carbon nanofiber-based luminol-biotin probe was synthesized for the sensitive chemiluminescence (CL) detection of a target protein by grafting luminol and biotin onto an oxidized carbon nanofiber. This carbon nanofiber was prepared by chemical vapor-deposition with methane in the presence of the Ni–Cu–MgO catalyst, which was followed by oxidization with HNO3–H2SO4to produce a carboxyl group on the surface of the nanofiber. The material was grafted with luminol and biotin by means of a standard carbodiimide activation of COOH groups to produce corresponding amides. The substance was water-soluble and thus could be utilized as a sensitive CL probe for a protein assay. The probe showed highly specific affinity towards the biotin-labeled antibody viaa streptavidin–biotin interaction. The detection limit for this model assay was approximately 0.2 pmol of the biotinized IgG spotted on a polyvinylidene fluoride (PVDF) membrane. Nonspecific binding to other proteins was not observed. Therefore, the synthesized carbon nanofiber-based CL probe may be useful for a sensitive and specific analysis of the target protein.