Preliminary X-Ray Data Analysis of Crystalline Cowpea Chlorotic Mottle Virus
- Resource Type
- Article
- Authors
- Speir, J.A.; Munshi, S.; Baker, T.S.; Johnson, J.E.
- Source
- Virology; March 1993, Vol. 193 Issue: 1 p234-241, 8p
- Subject
- Language
- ISSN
- 00426822; 10960341
Crystals of cowpea chlorotic mottle virus (CCMV) that diffract X-rays to 3.1 Å resolution were grown in a succinate-PEG solution buffered at pH 3.3. The crystals are in space group P212121with unit cell dimensions of a= 381.26 Å, b= 381.26 Å, and c= 408.59 Å. Four particles occupy the unit cell, placing a single virion in the crystallographic asymmetric unit. Diffraction intensities measured from 196 films collected at the Cornell High Energy Synchrotron Source accounted for 55% of the theoretically possible data to 3.2 Å. Unit cell dimensions and rotation function analyses of the X-ray data revealed that the particles were organized in a pseudo-tetragonal relationship with the pseudo-fourfold axis along the crystal caxis. Analysis of electron micrographs of two-dimensional crystals of CCMV revealed a remarkable similarity between these and planes of particles perpendicular to the crystallographic caxis in the three-dimensional crystal.