Aim:To determine the prevalence of New Delhi metallo-β-lactamase (NDM)-producing Gram-negative pathogens isolated from children’s samples. Materials & methods:Carbapenem-resistant clinical isolates (n = 117) were confirmed by VITEK®2 compact system, matrix-assisted laser desorption ionization–time of flight and multilocus sequence typing. MIC (μg/ml) of various antibiotics was determined by VITEK 2 compact system. Molecular characterization of the isolates was performed by PCR, DNA sequencing, PFGE and DNA hybridization. Results:Out of 117 carbapenemase producers, 37 (31.6%) and 29 (24.7%) were Klebsiella pneumoniaeand Acinetobacter baumannii, respectively. 72 (61.5%) isolates were NDM positive and among these 60, 9 and 3 were NDM-1, -5 and -7, respectively. Majority of the NDM-producing K. pneumoniaebelonged to ST11 and ST273 while most of the Escherichia colibelonged to ST405 and ST101. blaNDMwere mainly located on 150kb plasmids. MIC displayed high resistance against β-lactams drugs including carbapenems, and the most sensitive drugs were tigecycline and colistin. Conclusion:Dissemination of blaNDM-producing pathogens, particularly in children clinical settings, is a matter of great public health concern.