The article examines the co-existence of emerging bacterial pathogens Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum and Francisella tularensis in Ixodes ricinus ticks in Serbia. The researchers detected species-specific DNA sequences in using polymerase chain reaction (PCR) technique, including the rrf-rrl-rDNA intergenic spacer for B. burgdorferi s.l., p44/msp2 paralogs for A. phagocytophilum, and the 17 kDa lipoprotein gene, TLL4, for F. tularensis. In 16 ticks, simultaneous presence of A. phagocytophilum and different genospecies of B. burgdorferi s.l. complex was recorded while 15 and eight ticks had mixed infections with F. tularensis and genospecies of B. burgdorferi s.l. Three ticks, meanwhile, had triple pathogen species infections.