Serologic Evaluation of Human Microcystin Exposure.
- Resource Type
- Article
- Authors
- Hilborn, E. D.; Carmichael, W. W.; Soares, R. M.; Yuan, M.; Servaites, J. C.; Barton, H. A.; Azevedo, S. M. F. O.
- Source
- Environmental Toxicology; Oct2007, Vol. 22 Issue 5, p459-463, 5p
- Subject
- MICROCYSTINS
SERUM
HEMODIALYSIS patients
ENZYME-linked immunosorbent assay
BACTERIAL toxins
GAS chromatography/Mass spectrometry (GC-MS)
BUTYRIC acid
- Language
- ISSN
- 15204081
This article discusses the occurrence of microcystins in human serum samples among two kidney dialysis patients. The authors analyzed serum samples using gas chromatography/ mass spectrometry (GC/MS) to detect 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) and enzyme-linked immunosorbent assay (ELISA) methods to detect free microcystins. MMPB is derived from both protein-bound and free microcystins by chemical oxidation. They concluded that free microcystins appear to be a small but variable subset of total microcystins present in human serum.