Formaldehyde fixation is detrimental to actin cables in glucose-depleted S. cerevisiae cells.
- Resource Type
- Academic Journal
- Authors
- Vasicova P; Laboratory of Cell Reproduction, Institute of Microbiology of the CAS, v.v.i., Prague, Czech Republic.; Rinnerthaler M; Department of Cell Biology, Division of Genetics, University of Salzburg, Salzburg, Austria.; Haskova D; Laboratory of Cell Reproduction, Institute of Microbiology of the CAS, v.v.i., Prague, Czech Republic.; Novakova L; Laboratory of Cell Reproduction, Institute of Microbiology of the CAS, v.v.i., Prague, Czech Republic.; Malcova I; Laboratory of Cell Reproduction, Institute of Microbiology of the CAS, v.v.i., Prague, Czech Republic.; Breitenbach M; Department of Cell Biology, Division of Genetics, University of Salzburg, Salzburg, Austria.; Hasek J; Laboratory of Cell Reproduction, Institute of Microbiology of the CAS, v.v.i., Prague, Czech Republic.
- Source
- Publisher: Shared Science Publishers OG Country of Publication: Austria NLM ID: 101632887 Publication Model: Electronic Cited Medium: Print ISSN: 2311-2638 (Print) Linking ISSN: 23112638 NLM ISO Abbreviation: Microb Cell Subsets: PubMed not MEDLINE
- Subject
- Language
- English
- ISSN
- 2311-2638
Actin filaments form cortical patches and emanating cables in fermenting cells of Saccharomyces cerevisiae . This pattern has been shown to be depolarized in glucose-depleted cells after formaldehyde fixation and staining with rhodamine-tagged phalloidin. Loss of actin cables in mother cells was remarkable. Here we extend our knowledge on actin in live glucose-depleted cells co-expressing the marker of actin patches (Abp1-RFP) with the marker of actin cables (Abp140-GFP). Glucose depletion resulted in appearance of actin patches also in mother cells. However, even after 80 min of glucose deprivation these cells showed a clear network of actin cables labeled with Abp140-GFP in contrast to previously published data. In live cells with a mitochondrial dysfunction (rho 0 cells), glucose depletion resulted in almost immediate appearance of Abp140-GFP foci partially overlapping with Abp1-RFP patches in mother cells. Residual actin cables were clustered in patch-associated bundles. A similar overlapping "patchy" pattern of both actin markers was observed upon treatment of glucose-deprived rho + cells with FCCP (the inhibitor of oxidative phosphorylation) and upon treatment with formaldehyde. While the formaldehyde-targeted process stays unknown, our results indicate that published data on yeast actin cytoskeleton obtained from glucose-depleted cells after fixation should be considered with caution.
Competing Interests: Conflict of interest: The authors declare no conflict of interest.