Human neutrophils communicate remotely via calcium-dependent glutamate-induced glutamate release.
- Resource Type
- Academic Journal
- Authors
- Kopach O; Queen Square Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.; Sylantyev S; Queen Square Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.; Rowett Institute, University of Aberdeen, Ashgrove Road West, Aberdeen AB25 2ZD, UK.; Bard L; Queen Square Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.; Michaluk P; Queen Square Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.; BRAINCITY, Laboratory of Neurobiology, Nencki Institute of Experimental Biology PAS, 3 Pasteur Street, 02-093 Warsaw, Poland.; Heller JP; Queen Square Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.; School of Biotechnology, Dublin City University, Dublin 9, Ireland.; Gutierrez Del Arroyo A; Translational Medicine and Therapeutics, Queen Mary University of London, Mile End Road, London E1 4NS, UK.; Ackland GL; Translational Medicine and Therapeutics, Queen Mary University of London, Mile End Road, London E1 4NS, UK.; Gourine AV; Department of Neuroscience, Physiology and Pharmacology, University College London, Gower Street, London WC1E 6BT, UK.; Rusakov DA; Queen Square Institute of Neurology, University College London, Queen Square, London WC1N 3BG, UK.
- Source
- Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
- Subject
- Language
- English
Neutrophils are white blood cells that are critical to acute inflammatory and adaptive immune responses. Their swarming-pattern behavior is controlled by multiple cellular cascades involving calcium-dependent release of various signaling molecules. Previous studies have reported that neutrophils express glutamate receptors and can release glutamate but evidence of direct neutrophil-neutrophil communication has been elusive. Here, we hold semi-suspended cultured human neutrophils in patch-clamp whole-cell mode to find that calcium mobilization induced by stimulating one neutrophil can trigger an N-methyl-D-aspartate (NMDA) receptor-driven membrane current and calcium signal in neighboring neutrophils. We employ an enzymatic-based imaging assay to image, in real time, glutamate release from neutrophils induced by glutamate released from their neighbors. These observations provide direct evidence for a positive-feedback inter-neutrophil communication that could contribute to mechanisms regulating communal neutrophil behavior.
Competing Interests: The authors declare no competing interests.
(© 2023 The Authors.)