Rising atmospheric carbon dioxide (CO 2 ) and ozone (O 3 ) concentrations are the main global change drivers. Soil ectoenzymes play an important role in maintaining soil ecosystem services. Exploring the responses of soil ectoenzymes to elevated CO 2 and O 3 concentrations is important for combating global climate change. In this study, we simulated elevated CO 2 concentrations (+200 μmol·mol -1 , eCO 2 ), elevated O 3 concentrations (0.04 μmol·mol -1 , eO 3 ), and their combination (eCO 2 +eO 3 ) in open-top chambers (OTCs), and investigated the responses of rhizospheric soil ectoenzyme activities. The results showed that eCO 2 significantly increased the β-D-Glucosidase (βG) activity by 73.0%, and decreased that of polyphenol oxidase (PHO), peroxidase (PEO), and acid phosphatase (AP) by 48.9%, 46.6% and 72.9% respectively, but did not affect that of cellulose hydrolase (CBH) and β-N-Acetylglucosaminidase (NAG). eO 3 significantly reduced the activities of CBH and AP by 34.2% and 30.4%, respectively. The activities of PHO and AP were reduced by 87.3% and 32.3% under the eCO 2 +eO 3 compared with the control, respectively. Results of the principal coordinate analysis, permutation multivariate analysis of variance and redundancy analysis showed that both elevated CO 2 and O 3 significantly affected soil ectoenzyme activities, with stronger effects of elevated CO 2 than elevated O 3 . Root nitrogen content, root carbon to nitrogen ratio, soil microbial biomass carbon and nitrate nitrogen were the main drivers of soil ectoenzyme activities under elevated CO 2 and O 3 . Elevated O 3 could partially neutralize the effects of elevated CO 2 on soil ectoenzyme activities. In conclusion, elevated CO 2 and O 3 restrained the activities of most soil ectoenzyme, suggesting that climate change would threat soil ecosystem services and functions in the agroecosystem.