Fabrication of silver nanoparticles by a diethylene triamine-hyaluronic acid derivative and use as antibacterial coating.
- Resource Type
- Academic Journal
- Authors
- Martorana A; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: annalisa.martorana@unipa.it.; Pitarresi G; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: giovanna.pitarresi@unipa.it.; Palumbo FS; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: fabiosalvatore.palumbo@unipa.it.; Catania V; Department of Earth and Marine Sciences (DiSTeM), University of Palermo, Viale delle Scienze Ed. 16, 90128 Palermo, Italy. Electronic address: valentina.catania@unipa.it.; Schillaci D; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: domenico.schillaci@unipa.it.; Mauro N; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: nicolo.mauro@unipa.it.; Fiorica C; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: calogero.fiorica@unipa.it.; Giammona G; Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 32, 90123 Palermo, Italy. Electronic address: gaetano.giammona@unipa.it.
- Source
- Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 8307156 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-1344 (Electronic) Linking ISSN: 01448617 NLM ISO Abbreviation: Carbohydr Polym Subsets: MEDLINE
- Subject
- Language
- English
In this work a synthetic protocol for the functionalization of hyaluronic acid with diethylenetriamine (DETA) was standardized. HA-DETA derivatives were characterized by NMR and proton carbon correlation analysis HSQC and HMBC to confirm chemical structure. A selected derivative was used to set up a green fabrication procedure for HA-DETA capped silver nanoparticles with the aim to achieve a polymeric based coating with potential application in the treatment of medical devices associated infections. Data from UV-visible spectroscopy, electron scanning and transmission microscope (STEM), photoelectric spectroscopy (XPS) and rheological characterization were combined to characterize the HA-DETA/Ag nanocomposites. HA-DETA stabilized Ag nanoparticles (10-30 nm) were obtained through an UV accelerated production. The viability of MC3T3-E1 was analyzed with the aim of designing a cytocompatible antimicrobial coating. Antibacterial and antibiofilm activity of HA-DETA/Ag nanocomposites have been tested in vitro against Staphylococcus aureus and Pseudomonas aeruginosa both in culture plates than on titanium specimens.
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