Modeling the 3D Genome Using Hi-C and Nuclear Lamin-Genome Contacts.
- Resource Type
- Academic Journal
- Authors
- Paulsen J; EVOGENE, Department of Biosciences, Faculty of Natural Sciences, University of Oslo, Oslo, Norway. jonas.paulsen@ibv.uio.no.; Collas P; Department of Molecular Medicine, Faculty of Medicine, University of Oslo, Oslo, Norway.; Department of Immunology and Transfusion Medicine, Oslo University Hospital, Oslo, Norway.
- Source
- Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE
- Subject
- Language
- English
The three-dimensional (3D) organization of the genome is shaped by interactions with multiple structures within the nucleus, affecting gene expression outcomes. Technological breakthroughs in recent years have generated vast data reflecting various aspects of nuclear genome architecture in space and time. Integrating these datasets into comprehensive 3D genome models can reveal new insights into genome structure and regulation in normal and disease states. In this chapter, we provide a step-by-step guide on how to generate publication-ready integrated 3D genome models from (raw) Hi-C data and from lamin-genome (LAD) contacts.
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