Identification and analysis of cell wall glycan epitopes and polyphenol oxidase in pawpaw ( Asimina triloba [L.] Dunal) fruit pulp as affected by high pressure processing and refrigerated storage.
- Resource Type
- Academic Journal
- Authors
- Brannan RG; 1 School of Applied Health Sciences and Wellness, Ohio University, Athens, USA.; Faik A; 2 Environmental and Plant Biology, Ohio University, Athens, USA.; Goelz R; 3 Complex Carbohydrate Research Center, University of Georgia, Athens, USA.; Pattathil S; 3 Complex Carbohydrate Research Center, University of Georgia, Athens, USA.; 4 Mascoma LLC (Lallemand Inc.), Lebanon, New Hampshire, USA.
- Source
- Publisher: Sage Publications Country of Publication: United States NLM ID: 9889534 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1532-1738 (Electronic) Linking ISSN: 10820132 NLM ISO Abbreviation: Food Sci Technol Int Subsets: MEDLINE
- Subject
- Language
- English
This research explores the cell wall composition and polyphenol oxidase activity of two pawpaw ( Asimina triloba ) fruit varieties, Susquehanna and Green River Belle, that were subjected to high pressure processing and 45 days of refrigerated storage. We hypothesize that high pressure processing may inhibit enzymatic action responsible for pawpaw's deleterious postharvest tissue softening and browning. Glycome profiling uses mAb groupings that recognize 19 groups of glycan epitopes present in most major classes of cell wall glycans and was used to determine cell wall composition. Results show that both varieties have typical type I primary cell walls of flowering dicots. However, differences in the fine cell wall structure between the varieties can be inferred and the varieties behaved differently during refrigerated storage, likely indicating of a difference in cell wall-modifying enzymes present in the primary cell walls. High pressure processing treatment does not seem to be effective at eliminating polyphenol oxidase activity.