TBL38 atypical homogalacturonan-acetylesterase activity and cell wall microdomain localization in Arabidopsis seed mucilage secretory cells.
- Resource Type
- Academic Journal
- Authors
- Dauphin BG; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UT3-CNRS- INPT, Auzeville-Tolosane, France.; Ropartz D; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, PROBE Research Infrastructure, Nantes, France.; Ranocha P; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UT3-CNRS- INPT, Auzeville-Tolosane, France.; Rouffle M; UMR INRAE 1158 BioEcoAgro Biologie des Plantes et Innovation, Université de Picardie Jules Verne, Amiens, France.; Carton C; UMR INRAE 1158 BioEcoAgro Biologie des Plantes et Innovation, Université de Picardie Jules Verne, Amiens, France.; Le Ru A; Plateforme Imagerie-Microscopie, CNRS, Université de Toulouse, UT3-CNRS, Fédération de Recherche FR3450 - Agrobiosciences, Interactions et Biodiversité, Auzeville-Tolosane, France.; Martinez Y; Plateforme Imagerie-Microscopie, CNRS, Université de Toulouse, UT3-CNRS, Fédération de Recherche FR3450 - Agrobiosciences, Interactions et Biodiversité, Auzeville-Tolosane, France.; Fourquaux I; Centre de Microscopie Electronique Appliquée la Biologie (CMEAB), Faculté de Médecine Rangueil, UT3, Toulouse, France.; Ollivier S; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, PROBE Research Infrastructure, Nantes, France.; Mac-Bear J; INRAE, UR BIA, F-44316 Nantes, France.; INRAE, BIBS Facility, PROBE Research Infrastructure, Nantes, France.; Trezel P; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UT3-CNRS- INPT, Auzeville-Tolosane, France.; UMR INRAE 1158 BioEcoAgro Biologie des Plantes et Innovation, Université de Picardie Jules Verne, Amiens, France.; Geairon A; INRAE, UR BIA, F-44316 Nantes, France.; Jamet E; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UT3-CNRS- INPT, Auzeville-Tolosane, France.; Dunand C; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UT3-CNRS- INPT, Auzeville-Tolosane, France.; Pelloux J; UMR INRAE 1158 BioEcoAgro Biologie des Plantes et Innovation, Université de Picardie Jules Verne, Amiens, France.; Ralet MC; INRAE, UR BIA, F-44316 Nantes, France.; Burlat V; Laboratoire de Recherche en Sciences Végétales, Université de Toulouse, UT3-CNRS- INPT, Auzeville-Tolosane, France.
- Source
- Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
- Subject
- Language
- English
Plant cell walls constitute complex polysaccharidic/proteinaceous networks whose biosynthesis and dynamics implicate several cell compartments. The synthesis and remodeling of homogalacturonan pectins involve Golgi-localized methylation/acetylation and subsequent cell wall-localized demethylation/deacetylation. So far, TRICHOME BIREFRINGENCE-LIKE (TBL) family members have been described as Golgi-localized acetyltransferases targeting diverse hemicelluloses or pectins. Using seed mucilage secretory cells (MSCs) from Arabidopsis thaliana , we demonstrate the atypical localization of TBL38 restricted to a cell wall microdomain. A tbl38 mutant displays an intriguing homogalacturonan immunological phenotype in this cell wall microdomain and in an MSC surface-enriched abrasion powder. Mass spectrometry oligosaccharide profiling of this fraction reveals an increased homogalacturonan acetylation phenotype. Finally, TBL38 displays pectin acetylesterase activity in vitro . These results indicate that TBL38 is an atypical cell wall-localized TBL that displays a homogalacturonan acetylesterase activity rather than a Golgi-localized acetyltransferase activity as observed in previously studied TBLs. TBL38 function during seed development is discussed.
Competing Interests: The authors declare no competing interests.
(© 2024 The Author(s).)