Large field of view aberrations correction with deformable lenses and multi conjugate adaptive optics.
- Resource Type
- Academic Journal
- Authors
- Furieri T; Institute of Photonics and Nanotechnology, National Council of Research of Italy, Padova, Italy.; Department of Information Engineering, University of Padova, Padova, Italy.; Bassi A; Department of Physics, Politecnico di Milano, Milan, Italy.; Bonora S; Institute of Photonics and Nanotechnology, National Council of Research of Italy, Padova, Italy.
- Source
- Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101318567 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1864-0648 (Electronic) Linking ISSN: 1864063X NLM ISO Abbreviation: J Biophotonics Subsets: MEDLINE
- Subject
- Language
- English
Optical microscopes can have limited resolution due to aberrations caused by samples and sample holders. Using deformable mirrors and wavefront sensorless optimization algorithms can correct these aberrations, but the correction is limited to a small area of the field of view. This study presents an adaptive optics method that uses a series of plug-and-play deformable lenses for large field of view wavefront correction. A direct wavefront measurement method using the spinning sub-pupil aberration measurement technique is combined with correction based on the deformable lenses. Experimental results using fluorescence microscopy with a wide field and a light sheet fluorescence microscope show that the proposed method can achieve detection and correction over an extended field of view with a compact transmissive module placed in the detection path of the microscope. This method could improve the resolution and accuracy of imaging in a variety of fields, including biology and materials science.
(© 2023 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.)