Sulfobetaine-Phosphonate Block Copolymer Coated Iron Oxide Nanoparticles for Genomic Locus Targeting and Magnetic Micromanipulation in the Nucleus of Living Cells.
- Resource Type
- Academic Journal
- Authors
- Delille F; Laboratoire Physique et Etude des Matériaux, ESPCI-Paris, PSL Research University, CNRS, Sorbonne Université, UMR 8213, 10, rue Vauquelin, 75005 Paris, France.; Balloul E; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, 75005 Paris, France.; Hajj B; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, 75005 Paris, France.; Hanafi M; Sciences et Ingénierie de la Matière Molle, UMR 7615, ESPCI Paris PSL-CNRS-Sorbonne Université, 10 Rue Vauquelin, 75005 Paris, France.; Morand C; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, 75005 Paris, France.; Laboratoire Dynamique du Noyau, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR3664, 75005 Paris, France.; Xu XZ; Laboratoire Physique et Etude des Matériaux, ESPCI-Paris, PSL Research University, CNRS, Sorbonne Université, UMR 8213, 10, rue Vauquelin, 75005 Paris, France.; Dumas S; Institut Pierre-Gilles de Gennes, Institut Curie, Sorbonne Université, PSL Research University, 6 rue Jean Calvin, 75005 Paris, France.; Coulon A; Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR168, 75005 Paris, France.; Laboratoire Dynamique du Noyau, Institut Curie, PSL Research University, Sorbonne Université, CNRS UMR3664, 75005 Paris, France.; Lequeux N; Laboratoire Physique et Etude des Matériaux, ESPCI-Paris, PSL Research University, CNRS, Sorbonne Université, UMR 8213, 10, rue Vauquelin, 75005 Paris, France.; Pons T; Laboratoire Physique et Etude des Matériaux, ESPCI-Paris, PSL Research University, CNRS, Sorbonne Université, UMR 8213, 10, rue Vauquelin, 75005 Paris, France.
- Source
- Publisher: American Chemical Society Country of Publication: United States NLM ID: 101088070 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-6992 (Electronic) Linking ISSN: 15306984 NLM ISO Abbreviation: Nano Lett Subsets: MEDLINE
- Subject
- Language
- English
Exerting forces on biomolecules inside living cells would allow us to probe their dynamic interactions in their native environment. Magnetic iron oxide nanoparticles represent a unique tool capable of pulling on biomolecules with the application of an external magnetic field gradient; however, their use has been restricted to biomolecules accessible from the extracellular medium. Targeting intracellular biomolecules represents an additional challenge due to potential nonspecific interactions with cytoplasmic or nuclear components. We present the synthesis of sulfobetaine-phosphonate block copolymer ligands, which provide magnetic nanoparticles that are stealthy and targetable in living cells. We demonstrate, for the first time, their efficient targeting in the nucleus and their use for magnetic micromanipulation of a specific genomic locus in living cells. We believe that these stable and sensitive magnetic nanoprobes represent a promising tool to manipulate specific biomolecules in living cells and probe the mechanical properties of living matter at the molecular scale.